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200nm siinfekl peptide  (AnaSpec)

 
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    Structured Review

    AnaSpec 200nm siinfekl peptide
    (A) Schematic shows stereotactic coordinates for injection of syngeneic mouse glioma NS and GL26 cell line as well as the cell number, and expected median survival. AP: Anteroposterior; ML: Mediolateral; DV: Dorsoventral; MS: Median Survival. (B) OT-1 splenocytes are labelled with the CFSE dye and co-cultured with Gr-1high or <t>Gr-1low</t> <t>MDSCs</t> sorted from tumor. When OT-1 splenocytes divide due to SIINFIKLE stimulation, the resulting daughter cells incorporate half the number of cytoplasmic carboxyfluorescein- (CFSE) fluorescent molecules. As cells T cells divide in response to <t>SIINFEKL</t> stimulation, fluorescence intensity decreases exponentially. T cell proliferation can be assessed by measuring the corresponding decrease in the dye intensity via flow cytometry.
    200nm Siinfekl Peptide, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/200nm siinfekl peptide/product/AnaSpec
    Average 90 stars, based on 1 article reviews
    200nm siinfekl peptide - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Functional Assay to Assess T-cell Inhibitory Properties of Myeloid Derived Suppressor Cells (MDSCs) Isolated from the Tumor Microenvironment of murine Glioma Models"

    Article Title: Functional Assay to Assess T-cell Inhibitory Properties of Myeloid Derived Suppressor Cells (MDSCs) Isolated from the Tumor Microenvironment of murine Glioma Models

    Journal: Methods in enzymology

    doi: 10.1016/bs.mie.2019.05.047

    (A) Schematic shows stereotactic coordinates for injection of syngeneic mouse glioma NS and GL26 cell line as well as the cell number, and expected median survival. AP: Anteroposterior; ML: Mediolateral; DV: Dorsoventral; MS: Median Survival. (B) OT-1 splenocytes are labelled with the CFSE dye and co-cultured with Gr-1high or Gr-1low MDSCs sorted from tumor. When OT-1 splenocytes divide due to SIINFIKLE stimulation, the resulting daughter cells incorporate half the number of cytoplasmic carboxyfluorescein- (CFSE) fluorescent molecules. As cells T cells divide in response to SIINFEKL stimulation, fluorescence intensity decreases exponentially. T cell proliferation can be assessed by measuring the corresponding decrease in the dye intensity via flow cytometry.
    Figure Legend Snippet: (A) Schematic shows stereotactic coordinates for injection of syngeneic mouse glioma NS and GL26 cell line as well as the cell number, and expected median survival. AP: Anteroposterior; ML: Mediolateral; DV: Dorsoventral; MS: Median Survival. (B) OT-1 splenocytes are labelled with the CFSE dye and co-cultured with Gr-1high or Gr-1low MDSCs sorted from tumor. When OT-1 splenocytes divide due to SIINFIKLE stimulation, the resulting daughter cells incorporate half the number of cytoplasmic carboxyfluorescein- (CFSE) fluorescent molecules. As cells T cells divide in response to SIINFEKL stimulation, fluorescence intensity decreases exponentially. T cell proliferation can be assessed by measuring the corresponding decrease in the dye intensity via flow cytometry.

    Techniques Used: Injection, Cell Culture, Fluorescence, Flow Cytometry

    Reagents/Materials used in the protocol
    Figure Legend Snippet: Reagents/Materials used in the protocol

    Techniques Used: Modification, Red Blood Cell Lysis

    Splenocytes from OT-1 mice were extracted, labelled with CFSE dye, and stimulated in vitro with SIINFEKL peptide in the absence or presence of MDSCs for 4 days. Cells were stained with efluor 780 viability dye, Alexa fluor 700-CD45, Percp cy5.5-CD3, and Pacific Blue-CD8. Percentage of proliferating T cells were gated based on control condition (no SIINFEKL stimulation).
    Figure Legend Snippet: Splenocytes from OT-1 mice were extracted, labelled with CFSE dye, and stimulated in vitro with SIINFEKL peptide in the absence or presence of MDSCs for 4 days. Cells were stained with efluor 780 viability dye, Alexa fluor 700-CD45, Percp cy5.5-CD3, and Pacific Blue-CD8. Percentage of proliferating T cells were gated based on control condition (no SIINFEKL stimulation).

    Techniques Used: In Vitro, Staining, Control



    Similar Products

    200nm siinfekl peptide  (AnaSpec)
    90
    AnaSpec 200nm siinfekl peptide
    (A) Schematic shows stereotactic coordinates for injection of syngeneic mouse glioma NS and GL26 cell line as well as the cell number, and expected median survival. AP: Anteroposterior; ML: Mediolateral; DV: Dorsoventral; MS: Median Survival. (B) OT-1 splenocytes are labelled with the CFSE dye and co-cultured with Gr-1high or <t>Gr-1low</t> <t>MDSCs</t> sorted from tumor. When OT-1 splenocytes divide due to SIINFIKLE stimulation, the resulting daughter cells incorporate half the number of cytoplasmic carboxyfluorescein- (CFSE) fluorescent molecules. As cells T cells divide in response to <t>SIINFEKL</t> stimulation, fluorescence intensity decreases exponentially. T cell proliferation can be assessed by measuring the corresponding decrease in the dye intensity via flow cytometry.
    200nm Siinfekl Peptide, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/200nm siinfekl peptide/product/AnaSpec
    Average 90 stars, based on 1 article reviews
    200nm siinfekl peptide - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Schematic shows stereotactic coordinates for injection of syngeneic mouse glioma NS and GL26 cell line as well as the cell number, and expected median survival. AP: Anteroposterior; ML: Mediolateral; DV: Dorsoventral; MS: Median Survival. (B) OT-1 splenocytes are labelled with the CFSE dye and co-cultured with Gr-1high or Gr-1low MDSCs sorted from tumor. When OT-1 splenocytes divide due to SIINFIKLE stimulation, the resulting daughter cells incorporate half the number of cytoplasmic carboxyfluorescein- (CFSE) fluorescent molecules. As cells T cells divide in response to SIINFEKL stimulation, fluorescence intensity decreases exponentially. T cell proliferation can be assessed by measuring the corresponding decrease in the dye intensity via flow cytometry.

    Journal: Methods in enzymology

    Article Title: Functional Assay to Assess T-cell Inhibitory Properties of Myeloid Derived Suppressor Cells (MDSCs) Isolated from the Tumor Microenvironment of murine Glioma Models

    doi: 10.1016/bs.mie.2019.05.047

    Figure Lengend Snippet: (A) Schematic shows stereotactic coordinates for injection of syngeneic mouse glioma NS and GL26 cell line as well as the cell number, and expected median survival. AP: Anteroposterior; ML: Mediolateral; DV: Dorsoventral; MS: Median Survival. (B) OT-1 splenocytes are labelled with the CFSE dye and co-cultured with Gr-1high or Gr-1low MDSCs sorted from tumor. When OT-1 splenocytes divide due to SIINFIKLE stimulation, the resulting daughter cells incorporate half the number of cytoplasmic carboxyfluorescein- (CFSE) fluorescent molecules. As cells T cells divide in response to SIINFEKL stimulation, fluorescence intensity decreases exponentially. T cell proliferation can be assessed by measuring the corresponding decrease in the dye intensity via flow cytometry.

    Article Snippet: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Plate the CFSE labelled splenocytes at a density of 100,000 cells in 50μl in a 96 well round bottom plate (Thermo-fisher, MA) for the following conditions: list-behavior=enumerated prefix-word= mark-type=lower-alpha max-label-size=0 Condition 1: Unstimulated splenocytes (No SIINFEKL) Condition 2: Stimulated OT-1 splenocytes (+ SIINFEKL) Condition 3: Stimulated OT-1 splenocytes + 50μl Gr-1 high MDSCs Condition 4: Stimulated OT-1 splenocyte+ 50μl Gr-1 low MDSCs Prepare 1mL of 200nM SIINFEKL peptide (AnaSpec Inc, CA) in solution 2 to stimulate the OT-1 splenocytes.

    Techniques: Injection, Cell Culture, Fluorescence, Flow Cytometry

    Reagents/Materials used in the protocol

    Journal: Methods in enzymology

    Article Title: Functional Assay to Assess T-cell Inhibitory Properties of Myeloid Derived Suppressor Cells (MDSCs) Isolated from the Tumor Microenvironment of murine Glioma Models

    doi: 10.1016/bs.mie.2019.05.047

    Figure Lengend Snippet: Reagents/Materials used in the protocol

    Article Snippet: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Plate the CFSE labelled splenocytes at a density of 100,000 cells in 50μl in a 96 well round bottom plate (Thermo-fisher, MA) for the following conditions: list-behavior=enumerated prefix-word= mark-type=lower-alpha max-label-size=0 Condition 1: Unstimulated splenocytes (No SIINFEKL) Condition 2: Stimulated OT-1 splenocytes (+ SIINFEKL) Condition 3: Stimulated OT-1 splenocytes + 50μl Gr-1 high MDSCs Condition 4: Stimulated OT-1 splenocyte+ 50μl Gr-1 low MDSCs Prepare 1mL of 200nM SIINFEKL peptide (AnaSpec Inc, CA) in solution 2 to stimulate the OT-1 splenocytes.

    Techniques: Modification, Red Blood Cell Lysis

    Splenocytes from OT-1 mice were extracted, labelled with CFSE dye, and stimulated in vitro with SIINFEKL peptide in the absence or presence of MDSCs for 4 days. Cells were stained with efluor 780 viability dye, Alexa fluor 700-CD45, Percp cy5.5-CD3, and Pacific Blue-CD8. Percentage of proliferating T cells were gated based on control condition (no SIINFEKL stimulation).

    Journal: Methods in enzymology

    Article Title: Functional Assay to Assess T-cell Inhibitory Properties of Myeloid Derived Suppressor Cells (MDSCs) Isolated from the Tumor Microenvironment of murine Glioma Models

    doi: 10.1016/bs.mie.2019.05.047

    Figure Lengend Snippet: Splenocytes from OT-1 mice were extracted, labelled with CFSE dye, and stimulated in vitro with SIINFEKL peptide in the absence or presence of MDSCs for 4 days. Cells were stained with efluor 780 viability dye, Alexa fluor 700-CD45, Percp cy5.5-CD3, and Pacific Blue-CD8. Percentage of proliferating T cells were gated based on control condition (no SIINFEKL stimulation).

    Article Snippet: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Plate the CFSE labelled splenocytes at a density of 100,000 cells in 50μl in a 96 well round bottom plate (Thermo-fisher, MA) for the following conditions: list-behavior=enumerated prefix-word= mark-type=lower-alpha max-label-size=0 Condition 1: Unstimulated splenocytes (No SIINFEKL) Condition 2: Stimulated OT-1 splenocytes (+ SIINFEKL) Condition 3: Stimulated OT-1 splenocytes + 50μl Gr-1 high MDSCs Condition 4: Stimulated OT-1 splenocyte+ 50μl Gr-1 low MDSCs Prepare 1mL of 200nM SIINFEKL peptide (AnaSpec Inc, CA) in solution 2 to stimulate the OT-1 splenocytes.

    Techniques: In Vitro, Staining, Control